Chelex 100 is a chelating material from Bio-Rad used to purify other compounds via ion exchange. It is noteworthy for its ability to bind transition metal ions. It is a styrene-divinylbenzene co-polymer containing iminodiacetic acid groups. A concentrated solution of metals is obtained by eluting the resin with a small volume of 2 M nitric acid, which protonates the iminodiacetate groups. WebMar 18, 2024 · Chelex is a quick technique for DNA extraction from fungi. Summary. There are few protocols available for DNA extraction from fungi. Here we present four complementary protocols for extraction of genomic DNA from fungi. We quantify the efficacy of extractions and compare eight species from five filamentous fungal genera, including …
Optimization of Chelex 100 resin-based extraction of genomic …
WebNoun [ edit] chelex ( uncountable ) A resin used in ion exchange chromatography. WebOct 1, 2000 · Chelex is an ionic resin that can bind compounds which inhibit PCR (Singer-Sam et al., 1989). Compatibility, repeatability, reproducibility, and consistency of the PCR-fingerprinting patterns obtained by one chelex-based DNA extraction protocol were evaluated and compared with the classical phenol–chloroform DNA extraction procedure. paint scuff remover
Chelex Extraction - Forensic Science - Barnard Health Care
WebFeb 3, 2006 · A simple, rapid Chelex resin column method has been developed for the determination of metal speciation in natural water samples. A water sample (pH 6–8.2) was pumped through a small plug of the Ca-form of Chelex 100 at a flow rate of 48 ± 4 mL/min.The flow regime was optimised to give the shortest possible contact time (0.25 s) … WebProduct name : Chelex 100 sodium form Product Number : C7901 Brand : Sigma CAS-No. : 11139-85-8 1.2 Relevant identified uses of the substance or mixture and uses advised against Identified uses : Laboratory chemicals, Synthesis of substances 1.3 Details of the supplier of the safety data sheet Company : Sigma-Aldrich Inc. WebChelex 100 and COVID-19. or InstaGene Matrix. Chelex 100 Resin is a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions. The carboxylic acid groups of Chelex 100 Resin classify it as a weak cation exchange resin, but it differs from other exchangers in this … sugar and hyperactivity in children