WebDec 1, 2024 · In order to further quantify the effect of Ce6-PDT on autophagy, autophagy-related protein Atg5 was measured using western blotting. As shown in Fig. 3B, a dose-dependent increase in Atg5 levels was observed in the Ce6-PDT cells compared to the control and irradiation-only cells. These data suggest that Ce6-PDT induces autophagy … WebNational Center for Biotechnology Information
Dual Inhibition of PIK3C3 and FGFR as a New Therapeutic …
WebMay 17, 2024 · We identified a novel and direct interaction between ATG5, autophagy protein, and Ku70, an NHEJ repair mechanism protein. ATG5-Ku70 interaction predominantly occurs in the nuclei. This interaction ... WebATG5 (C-1): sc-133158 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND In yeast, autophagy is an essential process for survival during nutrient starva-tion and cell differentiation. The process of autophagy is characterized as a tax at home
Endothelial autophagy deficiency induces IL6 - dependent …
WebAug 7, 2024 · Briefly, 1 mg of Dynabeads was conjugated to anti-FADD (Santa Cruz, sc-6036), anti-ATG5 (CST, 8540) antibody, goat IgG control (Santa Cruz, sc-2028) or rabbit IgG (Jackson ImmunoResearch, Ely, UK, 011-000-003) overnight at 4 °C. Dynabeads were washed in ice-cold NP-40 lysis buffer and combined with cell lysates on a rotor at 4 °C … WebHere, using mice with genetic ablation of the essential Atg5 (autophagy related 5) or Atg7 (autophagy related 7) in their epidermis to inhibit autophagy, we show that keratinocyte autophagy regulates wound healing in mice. Wounding induces the expression of autophagy genes in mouse skin. Epidermis-specific autophagy deficiency inhibits wound ... WebSep 20, 2024 · Hypoxia stimulation or ectopic FOXO3 overexpression obviously increased the luciferase activities driven by the Atg5 or Atg16L promoter, whereas FOXO3 silencing led to a notable decrease in Atg5 and Atg16L reporter activities (Figure 5e), suggesting FOXO3 is a potential transcription factor for Atg5 and Atg16L expression. tax at home edmonton