Phnshp45

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August undefined, 2024

Webb16 okt. 2024 · Newport strains ASGD2 to pHNSHP45-2 revealed that this plasmid aligned very well with pHNSHP45-2 except that a region containing the tra genes, which were responsible for conjugation, was absent ... Webb15 feb. 2024 · pHNSHP45 KP347127 I2 E. coli Pig China [1] pEC019 KY471145 I2 E. coli Chicken Korea [2] pEC111 KY471146 IncX4 E. coli Pig Korea [2] pEC006 KY471144 I2 E. …

Microorganisms Free Full-Text Mobile Colistin Resistance …

Webb24 maj 2016 · In particular, the pHNSHP45 3 is the only one mcr-1-carring plasmid with known genome sequence prior to our study. Unlike the other mcr-1 -positive plasmid … Webb开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 gerald d marks obituary

Possible genetic events producing colistin resistance gene mcr-1

Webb10 jan. 2024 · The removal of the mcr-1 gene from plasmid pHNSHP45 not only led to reduced resistance to colistin but also resulted in a significant change in the membrane permeability of E. coli. Unexpectedly, the removal of the mcr-1 gene increased cell viability under high osmotic stress conditions (e.g., 7.0% NaCl) and led to increased resistance to … WebbGao, R., Wang, Q., Li, P., Li, Z., & Feng, Y. (2016). Genome sequence and characteristics of plasmid pWH12, a variant of themcr-1-harbouring plasmid pHNSHP45, from ... WebbThe PCR based mapping of mcr-1 genetic context in our isolates revealed that almost all amplified genes were similar to pHNSHP45, except the truncated tnpA gene, missing IsApl1. Globally, plasmid analysis of various Incompatibility types has shown that IsApl1 is usually absent in IncX4 type plasmid while in Incl2 type plasmid, it is either absent or … christina alsop

Associations between human bacterial pathogens and ARGs are …

#Colistin #resistance #gene #mcr1 and pHNSHP45 #plasmid in …

WebbThe global dissemination of the mobilized colistin resistance gene, mcr-1, threatens human health. Recent studies by our group and others have shown that the withdrawal of … Webbthat plasmid pHNSHP45 exhibited an in vivo transfer rate between different E. coli strains (measured at 10–1 to 10–3 per recipient) (7), a feature that could contribute to the suc - cessful dissemination of the mcr-1 gene. Similarly, in our study, we can also confirm the transfer of the mcr-1 gene christina allen yale orthoWebbEuropean Centre for Disease Prevention and Control (ECDC), European Food Safety Authority (EFSA) and European Medicines Agency (EMA). ECDC/EFSA/EMA first joint report on the integrated analysis of the consumption of antimicrobial agents and occurrence of antimicrobial resistance in bacteria from humans and food-producing animals. christina alsop a.p.n.npi

"Webb60 kb in-size, and were similar to pHNSHP45 (Fig. 1), the original sequenced mcr-1 plasmid published in 2015 [5]. The three IncI2 plasmids shared a common plasmid backbone, however, in the case of pPC11 the mcr-1 cas-sette [17] was located in an inverted orientation com-pared to the others (data not shown). Additionally, the " - Phnshp45

Phnshp45

Identifying early signs: the example of antimicrobial resistance

Webb7 jan. 2016 · The mcr-1-associated plasmid in the study by Liu and colleagues, pHNSHP45, is an approximate 64 kb IncI plasmid with substantial sequence homology (≥80% … WebbOne mcr-1-carrying Salmonella enterica serovar Indiana strain D90, was identified from 1320 Salmonella enterica isolates from poultry slaughterhouse in 2012 in China. The objective of this study was to verify the transferability of the mcr-1 gene and also completely characterize the sequence of the strain at the whole-genome level. Broth …

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Webb27 feb. 2024 · Fitness Effects of mcr-1 Gene and mcr-1 Plasmids in Vitro. The impact of plasmids pHNSHP45, pHNSHP45-2, pHNSHP23, pHNSHP17 and pHNSHP24 on host fitness was evaluated by direct competition assays of plasmid-carrying E. coli DH5α against plasmid-free E. coli DH5α. The transformants with pHNSHP45(IncI2) or … WebbThe Lancet Infectious Diseases 2016 16, 283-284DOI: (10.1016/S1473-3099(16)00012-8) 21 Blast comparison with pHNSHP45 showed 100% similarity only in a short, 2604 bp …

WebbPlasmid Transformation Assays. The competent cells including E. coli DH5α containing pUC19-mcr-1 and E. coli C600+pHNSHP45 were prepared and used for transformation … Webb6 juli 2024 · Relative to the paradigm mcr-1-harboring IncI2-type plasmid pHNSHP45, pGD17-2 exhibited 51% coverage and 99% identity and possessed two insertion sequences (IS629 and IS1294) in the nikB gene, as well as an additional IS629-mediated insertion sequence (~56 kb) in the pilO gene (Fig. 1 and Additional file 2: Figure S2).

WebbThe global dissemination of the mobilized colistin resistance gene, mcr-1, threatens human health. Recent studies by our group and others have shown that the withdrawal of colistin as a feed additi... Webb6 mars 2024 · 35 China right now, we applied its 2 surrogate plasmids, pHNSHP45 and pDJB-3 36 (Fig. 1A), in the conjugation assays. In general consistency with an 37 observation of Zhi et al. 29, the efficiency of pHNSHP45-2 transfer is calculated 38 to be 3.7x10-5 in our experiment of conjugations. In contrast, pDJB-3 can’t

Webb10 jan. 2024 · The plasmid pHNSHP45 was extracted from E. coli K12 C600/pHNSHP45 using the EZNA ® plasmid mini kit I (Omega) and then electroporated into the E. coli K12 …

Webb29 juni 2024 · 2.2. mcr-1 Inhibits pHNSHP45 Transfer through Conjugation mcr-1 is carried by the plasmid pHNSHP45, hereafter referred to as the mcr-1 plasmid. To examine whether the mcr-1 gene contributes to the transfer of pHNSHP45, we replaced the mcr-1gene with a kanamycin-resistant cassette (KmR) on pHNSHP45 to obtain the pAC22 gerald docherty glasgowWebbColistin resistance gene mcr-1 and pHNSHP45 plasmid in human isolates of Escherichia coli and Klebsiella pneumoniae. Colistin resistance gene mcr-1 and pHNSHP45 plasmid … gerald d moulds rate my professorWebbHello, I am looking for the copy number of the plasmid pHNSHP45 in E.coli isolates. The plasmid should be present. However, it was not identified by plasmidseeker. Therefore, I … gerald dixon attorney delawareWebb27 juni 2024 · For instance, a 222.3-kb section of pWJ1 (85.4% of the total sequence) exhibited high similarity to the corresponding region of the 251-kb plasmid pHNSHP45-2 … christina alsop aprnWebb21 mars 2024 · The emergence and spread of antibiotic resistance genes (ARGs) have imposed a serious threat on global public health. Horizontal gene transfer (HGT) via plasmids is mainly responsible for the spread of ARGs, and conjugation plays an important role in HGT. The conjugation process is very active in vivo and its effect on the spreading … christina alsop renownWebb28 nov. 2016 · The insertion sequence ISApl1 occurs in both pHNSHP45 and pA31-12 is absent in the plasmid pE15017, which is validated by PCR detection coupled with Sanger … christina altomare yonkersWebb24 maj 2016 · In particular, the pHNSHP45 3 is the only one mcr-1-carring plasmid with known genome sequence prior to our study. Unlike the other mcr-1 -positive plasmid … gerald domitrovic attorney wichita ks