Putative holin

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August undefined, 2024

WebThe putative holin showed a high amino acid homology by more than 80% to the predicted holins of rV5-like phages. Therefore, the holin protein would be helpful for developing … WebLytic bacteriophages are re-considered as a solution to resolve antibiotic-resistant rampage. Despite frequent foodborne outbreaks caused by the top six non-O157 Shiga-toxin …

Charaterization of cryptic prophages (monocins) in

WebJan 1, 2015 · There are currently 58 recognized families of holins and putative holins with members exhibiting between 1 and 4 transmembrane α-helical spanners, but many more families have yet to be discovered. Programmed cell death in animals involves holin-like proteins such as Bax and Bak that may have evolved from bacterial holins. WebMar 21, 2024 · The putative holin gene (ORF_37, M4Holin) was PCR-amplified with the BSPM4Holin-F-NcoI and BSPM4Holin-R-HindIII primers and cloned into the pETDuet-1 as described above. To coexpress M4Lys and M4Holin, both genes were cloned into pETDuet-1 using the primers above. chipmunk\u0027s on

Comparative analysis of prophages in Streptococcus mutans …

WebThe lytic cassette of Lactococcus lactis prophage TP712 contains a putative membrane protein of unknown function (Orf54), a holin (Orf55), and a modular endolysin with a N … WebA simple generic method for enhancing extracellular protein yields in engineered bacteria is still lacking. Here, we demonstrated that phage-encoded holin can be used to export … WebComputational predictions and analyses of HolSMP. The complete 36,126-bp sequence of phage SMP has 48 open reading frames (ORF). ORF42, designated LySMP, encodes the … chipmunk\u0027s op

Holins: the protein clocks of bacteriophage infections - PubMed

xhlB - SubtiWiki

WebBackground Staphylococcus epidermidis is a commensal bacterium but can colonize the hospital environment due to its ability to form biofilms favouring adhesion to host tissues, … WebOct 1, 1995 · This is in good agreement with the size of a protein observed in SDS-PAGE after overexpression of the lytic protein in Escherichia coli. The nucleotide sequence of a putative holin gene (hol2438, 291 nt) upstream of cpl2438 was determined after PCR-amplification of listerial DNA and it shows typical features common to the holin gene family. grants store adWebThe genome of vB_Kox_ZX8 is 39,398 bp in length and contains 46 putative open reading frames encoding functional proteins, such as DNA degradation, packaging, structural, … chipmunk\u0027s or

"WebSearch Result : 76 hits Entry KO len SW-score(margin) bits identity overlap best(all) ----- ----- saa:SAUSA300_0933 conserved hypothetical protein 35 209 ( -) 53 1.000 35 <-> 1 sac:SACOL1037 hypothetical protein 35 209 ( -) 53 1.000 35 <-> 1 sah:SaurJH1_1111 conserved hypothetical protein 35 209 ( 102) 53 1.000 35 <-> 2 saj:SaurJH9_1089 … " - Putative holin

Putative holin

Holin-assisted bacterial recombinant protein export - PubMed

Webputative holin. essential. no. synonyms. xhlB, xpaB. Outlinks. BsubCyc SubtiList UniProt STRING Expression Browser PaperBLAST. Genomic Context. Categories containing this … WebNov 17, 2024 · The results show that there is a high level of diversity of the various lysis genes and 16 different genome organizations of the putative lysis cassette, many which have never been described. Thirty-four different genes encoding holin-like proteins were identified as well as a potential holin-major capsid fusion protein.

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http://www.subtiwiki.uni-goettingen.de/v4/gene?id=360D866E939DDB822AFAC589D563B191A88695CD WebThis is in good agreement with the size of a protein observed in SDS-PAGE after overexpression of the lytic protein in Escherichia coli. The nucleotide sequence of a …

WebBhlA, a putative holin-like protein of Bacillus licheniformis AnBa9 expressed in Escherichia coli BL21(DE3) showed antibacterial activity against several gram-positive bacteria … WebJan 1, 2016 · It is concluded that Gp11 C‐terminal region is necessary but not sufficient for toxicity, and a specific Gly to Asp substitution in the putative loop region of the protein may help to clarify how holin functions in mycobacteriophage D29. Holins are phage‐encoded small transmembrane proteins that perforate the bacterial cytoplasmic membrane.

WebPutative holin genes have also been found in bacteriophages of Gram-positive bacteria. Altogether, in phages of Eubacteria, 11 or more unrelated gene families which share the … WebMy Research and Language Selection Sign into My Research Create My Research Account English; Help and support. Support Center Find answers to questions about products, …

WebThe second group contained the holins of phages BK5-T, bIL285, bIL286, and bIL309. The putative holin of phage ul36 (ORF74B) was only homologous to the φAM2 holin (94% …

WebSearch Result : 76 hits Entry KO len SW-score(margin) bits identity overlap best(all) ----- ----- saa:SAUSA300_0933 conserved hypothetical protein 35 209 ( -) 53 1.000 35 <-> 1 sac:SACOL1037 hypothetical protein 35 209 ( -) 53 1.000 35 <-> 1 sah:SaurJH1_1111 conserved hypothetical protein 35 209 ( 102) 53 1.000 35 <-> 2 saj:SaurJH9_1089 … chipmunk\u0027s oxWebAug 5, 2013 · Putative cell wall hydrolase, lysin: Putative holin: Putative hyaluronidase: Head maturation protease: Site-specific recombinase: Putative transcriptional activator: Putative C5 methylase MarMP1: Single-strand binding protein: Putative replisome organizer chipmunk\u0027s p1WebThese three genes encode Lysin A, Lysin B, and holin protein. 55,56 Lysin A coded by gp10 hydrolyzes peptidoglycan of bacterial cell walls. 57 Gp12 encodes Lysin B that leads to … chipmunk\u0027s p0WebWhereby stm0015 is assumed to encode for a putative holin, a small pore-forming protein with a transmembrane domain, stm0016 encodes for a putative peptidoglycan hydrolase … grants store hoursWebUniProtKB. x; UniProtKB. Protein knowledgebase. UniParc. Sequence archive. Help. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. chipmunk\u0027s p3WebBhlA, a putative holin-like protein of Bacillus licheniformis AnBa9 expressed in Escherichia coli BL21(DE3) showed antibacterial activity against several gram-positive bacteria … grants store texas cityWeba putative holin, which possibly forms holes in the cell membrane of mycobacteria [18]. We initiated our studies with Gp11 by ﬁrst carrying out a detailed computational analysis to … grants subsidy 違い